2017 Fiscal Year Final Research Report
Global phosphoproteome analysis of IgG4-related sclerosing cholangitis
Project/Area Number |
15K08345
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Human pathology
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Research Institution | Kobe University |
Principal Investigator |
Zen Yoh 神戸大学, 医学研究科, 特命教授 (90377416)
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Project Period (FY) |
2015-04-01 – 2018-03-31
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Keywords | IgG4 / 硬化性胆管炎 / プロテオーム / リン酸化 / 自己免疫性膵炎 / マクロファージ |
Outline of Final Research Achievements |
The tissue phosphoproteomic examination of IgG4-related sclerosing cholangitis (IgG4-SC) and primary sclerosing cholangitis (PSC) revealed that the expression profiles of phosphopeptides discriminated IgG4-SC from PSC better than those of non-phosphopeptides. In the pathway analysis, three immunological pathways activated in IgG4-SC appeared to be all B-cell- or immunoglobulin-related. On immunostaining, two highly up-regulated immunological markers in IgG4-SC were expressed mainly in M2-type macrophages, suggesting increased phagocytotic activity induced by the IgG-Fc-gamma receptor interaction. In addition, those molecules were also strongly expressed in other organ manifestations of IgG4-related disease. In conclusion, this study highlighted crucial roles of B cells and macrophages in IgG4-SC, and also identified potential tissue diagnostic markers for IgG4-related disease.
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Free Research Field |
人体病理学
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