The positive marker identification of tumor-initiating cells and the dynamic analyses of them in lymphoplasmacytic lymphoma (LPL)

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K08363
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Human pathology
• Research Institution: Osaka University
• Principal Investigator: WADA Naoki 大阪大学, 医学系研究科, 助教 (80521731)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 血液 / リンパ腫 / 低酸素 / ケモカインレセプター / 形質細胞分化

Outline of Final Research Achievements

MWCL-1 was established as LPL cell line. MWCL-1 cells were classified into three subpopulations by flow cytometric analyses using anti-CD20 and anti-CD138 antibodies: CD20－CD138－, CD20＋CD138－, and CD20＋CD138＋ cells. We searched for markers preferentially expressed in CD20－CD138－ cells of MWCL-1 (the candidates for tumor-initiating cells of LPL), and the chemokine receptor CXCR7 was isolated. We also searched for favorable microenvironments for their proliferation, and we found that hypoxia and CXCL12-CXCR7 signaling appeared to be favorable microenvironments. We then examined the effect of plasma-activated medium (PAM) on LPL cell line, and we found that PAM induced plasma cell (CD138 positive) differentiation, which appeared to be mediated by PRDM1α (the transcription factor promoting plasma cell differentiation).

Free Research Field

悪性リンパ腫