Roles of Girdin in neural and cancer cell migration along vessels

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K08399
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Experimental pathology
• Research Institution: Nagoya University
• Principal Investigator: Asai Naoya 名古屋大学, 医学系研究科, 准教授 (80273233)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 脳神経発生 / チロシンリン酸化 / GEF活性 / 遺伝子改変動物 / 細胞移動

Outline of Final Research Achievements

Girdin play important roles for brain development at hippocampus, olfacrory blub/RMS, anterior commissure and GABAergic neurons of cortex. Girdin has two tyrosine phosphorylation sites and GEF domain, but these functions in vivo have not been clear.
To study the function of Girdin tyrosine phosphorylation at Y1764 and Y1798, we generated Girdin Y1764,1798F mutant mice. Mutant mice show no obvious phenotype at hippocampus and olfactory bulb/RMS, which may indicate the less important roles of Girdin tyrosine phosphorylation for cell motility at developing brain. In addition, we generate Girdin F1696A mutant mice, which would lack GEF activity of Girdin. Mutant mice show abnormal brain structure at hippocampus and olfactory blub/RMS resembling Girdin knockout mice. Mutant mice also show milder phenotypes at anterior commissure and distribution of GABAergic neurons in cortex. These data suggest importance of Girdin GEF activity for cell motility.

Free Research Field

実験病理