2017 Fiscal Year Final Research Report
Identification of novel genes responsible for malignant progression in renal cell cancer
| Project/Area Number |
15K08405
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Experimental pathology
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| Research Institution | Oita University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | 腎細胞癌 / 悪性化 / 責任遺伝子 / WDR20 / Sav1 |
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| Outline of Final Research Achievements |
We had identified the genes specifically down-regulated in malignant renal cell carcinomas. Among them, we focused on WDR20 and Sav1 genes and analyzed their functions in the renal cancer cells. When WDR20 gene were introduced into the cancer cells, cell growth was significantly inhibited and apoptosis was induced. Therefore, WDR20 would function as tumor suppressor gene in renal cell carcinomas.
We established a Sav1 conditional knockout mouse which lost Sav1 expression specifically in renal tubes. The mice showed hyperproliferation of renal tubes and variations in cell size and nuclear shape of the epithelium, suggesting that Sav1 is required for the maintenance of growth, nuclear size and structure of renal tubules under physiological conditions, and its deficiency leads to the acquisition of enhanced proliferation of renal epithelial cells.
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| Free Research Field |
病理学
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