Molecules involved in intracellular trafficking of botulinum neurotoxin binding protein NTNHA

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K08474
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Bacteriology (including mycology)
• Research Institution: Tokyo University of Agriculture
• Principal Investigator: Sagane Yoshimasa 東京農業大学, 生物産業学部, 教授 (00624660)
• Co-Investigator(Renkei-kenkyūsha): NIWA Koichi 東京農業大学, 生物産業学部, 教授 (20301012) ; MUTOH Shingo 鎌倉女子大学, 家政学部, 講師 (20749890) ; SUZUKI Tomonori 東京農業大学, 生命科学部, 准教授 (90453836)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: ボツリヌス食中毒 / 細胞内輸送 / 経口毒素

Outline of Final Research Achievements

Nontoxic nonhemagglutinin (NTNHA) is a protein that binds to botulinum neurotoxin (BoNT), a causative agent of botulism food poisoning. NTNHA is known to increase oral toxicity of toxin by protecting BoNT from digestive juices, but its function is remained unclear. This study aims to elucidate the molecules involved in intracellular transport of NTNHA. The results of this study suggested that "actin-myosin" system is involved in NTNHA intracellular transport. Also, vesicles involved in the intracellular transport of NTNHA were revealed. The results of this study are expected to greatly contribute to the elucidation of the invasion mechanism of botulinum toxin into the body.

Free Research Field

分子生物学