2017 Fiscal Year Final Research Report
Development of diagnostic methods for autoimmune dyslipidemia
Project/Area Number |
15K08613
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Laboratory medicine
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Research Institution | Osaka University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
木原 進士 大阪大学, 医学系研究科, 教授 (20332736)
|
Project Period (FY) |
2015-04-01 – 2018-03-31
|
Keywords | 自己免疫 / 脂質異常症 |
Outline of Final Research Achievements |
It has been estimated that autoimmunity is involved in the pathogenesis for severe dyslipidemia with unknown origin, but its diagnosis is not easy. In this study, we have developed a system to detect the anti-apolipoprotein (apo) C-II autoantibodies, which lead to severe hypertriglyceridemia. As a first screening test, we measured the serum anti-apo C-II autoantibody levels against the synthetic partial peptides of apo C-II by ELISA (Enzyme-Linked ImmunoSorbent Assay). Among the five selected patients of hypertriglyceridemia with high autoantibody levels, we could identify the autoantibodies associated with the endogenous apo C-II by immunoprecipitation/immunoblot method in two patients. Also, we found that the serum levels of IgA class autoantibody might be related with the stage of diabetic nephropathy.
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Free Research Field |
臨床検査
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