The development of new pathologic diagnosis system using HLA-F expression on tumor

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K08650
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Nara Medical University
• Principal Investigator: Ouji-Sageshima Noriko (下嶋典子) 奈良県立医科大学, 医学部, 助教 (30398432)
• Co-Investigator(Kenkyū-buntansha): 伊藤 利洋 奈良県立医科大学, 医学部, 教授 (00595712) ; 石谷 昭子 奈良県立医科大学, 医学部, 非常勤講師 (40112544)
• Co-Investigator(Renkei-kenkyūsha): Obayashi Chiho 奈良県立医科大学, 医学部, 教授 (90223940) ; Nakai Tokiko 奈良県立医科大学, 医学部, 助教 (00619538)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 免疫染色 / 腫瘍 / 診断 / HLA-F

Outline of Final Research Achievements

We have investigated the HLA-F expression on tumor tissues to develop the new method for the tumor diagnosis using HLA-F. HLA-F is one of HLA class I molecules, but it has low polymorphism and has restricted expression pattern. The staining method of HLA-F was optimized for the formalin fixed-paraffin embedded tumor tissues. HLA-F expression was detected all poorly-differentiated colon cancer, which we have tested. Especially, the strong HLA-F expression was detected on 75% of poorly-differentiated colon cancer and 26% of moderately- or well-differentiated colon cancer. On the analysis of HLA-F mRNA on tumor cell lines, HLA-F mRNA was upregulated following with tumor malignancy. These results indicated that HLA-F expression on tumor was associated with tumor malignancy. However we need more investigation to show clearly the association between HLA-F and tumor progression and to show utility of new system for tumor diagnosis using HLA-F.

Free Research Field

腫瘍免疫