Elucidation of the drug-resistance acquisition mechanism in Mycobacterium tuberculosis to be multidrug and extremely-multidrug resistant and identification of those possible genetic markers.

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K08724
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Epidemiology and preventive medicine
• Research Institution: Hokkaido University
• Principal Investigator: Nakajima Chie 北海道大学, 人獣共通感染症リサーチセンター, 准教授 (60435964)
• Co-Investigator(Renkei-kenkyūsha): SUZUKI Yasuhiko 北海道大学, 人獣共通感染症リサーチセンター, 教授 (90206540)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 結核 / 多剤耐性 / 遺伝子解析 / 型別 / 疫学

Outline of Final Research Achievements

Sequential acquisition of mutations in drug-resistance associating genes causes an emergence of multidrug-resistant Mycobacterium tuberculosis (MDR-MTB). The Beijing genotype of MTB is known to be associating with MDR, and its prevalence among MDR-MTB population was significantly higher than that in non-MDR MTB in three TB high-burden countries; Myanmar, Nepal and Thailand. The majority of the Beijing type isolates belonged to the “modern” subtype and most of them had putative KatG S315T and RpsL K43R mutations suggesting those were isoniazid and streptomycin resistant. Those genetic characteristics can be used as a marker of transmissible MDR-MTB; thus, we have developed simple detection methods including a multiplex PCR to identify the “modern” type of the Beijing lineage and a line probe assay to diagnose isoniazid resistance.

Free Research Field

細菌学、感染症学