Development of minimal-invasive and quantitative analytical method for nitrated DNA damage, and its application to risk assessment of inflammation-related carcinogenesis

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K08787
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Hygiene and public health
• Research Institution: Suzuka University of Medical Science
• Principal Investigator: Kawanishi Shosuke 鈴鹿医療科学大学, 薬学部, 教授 (10025637)
• Co-Investigator(Kenkyū-buntansha): 大西 志保 鈴鹿医療科学大学, 薬学部, 助教 (80511914)
• Co-Investigator(Renkei-kenkyūsha): MA Ning 鈴鹿医療科学大学, 医療科学研究科, 教授 (30263015) ; TABATA Tsutomu 三重大学, 医学研究科, 准教授 (40252358) ; IIJIMA Katsunori 秋田大学, 医学研究科, 教授 (60375003)
• Research Collaborator: MURATA Mariko 三重大学大学院, 医学研究科, 教授 (10171141)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: がん予防 / 炎症 / DNA損傷

Outline of Final Research Achievements

Chronic infection and inflammation have been recognized as important factors for carcinogenesis. Inflammation is estimated to contribute to approximately 25 % of human cancers. It is urgent problem to be solved, the risk assessment in early phase and prevention of inflammation-related carcinogenesis. We have reported that 8-nitroguanine, nitrative DNA damage specifically generated under inflammation, is generated in cancerous site before carcinogenesis. Therefore, 8-nitroguanine is hopeful marker for risk assessment of inflammation-related carcinogenesis. However, it is difficult to analyze 8-nitroguanine with quantitativity and minimal-invasiveness. In this study, we successed to confirm the amount of 8-nitroguanine quantitatively with using high performance liquid chromatography coupled with an electrochemical detector (HPLC-ECD).

Free Research Field

衛生学