Development of the analytical method to quantify toxicological substances in formalin-fixed tissue

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K08874
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Legal medicine
• Research Institution: Ehime University
• Principal Investigator: Asano Migiwa 愛媛大学, 医学系研究科, 教授 (90283879)
• Co-Investigator(Kenkyū-buntansha): 上野 易弘 神戸大学, 医学研究科, 教授 (30184956)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 薬物中毒 / 薬物定量分析 / ホルマリン固定 / 肝臓 / UPLC-MS/MS

Outline of Final Research Achievements

The goal of this study was to consider whether formalin-fixed human tissues for pathological examination were useful as substitutes for conventional specimens in toxicological analysis. As a first step, we developed UPLC-MS/MS analytical methods to simultaneous analysis for 60 drugs. Some psychotropic drugs were markedly depleted to varying degrees in formalin-fixed porcine liver homogenates. In forensic practice case, the drug was detected in formalin-fixed tissues, only if the concentration of the drug in blood was significantly high. It was suggested that in forensic practice formalin-fixed organs was partially useful for toxicological examination.

Free Research Field

法医学