2017 Fiscal Year Final Research Report
Molecular dissection of Cancer microenvironment-related genes
| Project/Area Number |
15K08973
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Sapporo Medical University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | 微小環境 / 大腸がん / 遺伝子発現 |
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| Outline of Final Research Achievements |
We identified genes and splicing variant which were altered in the tumor endothelial cells. We then validated the results by RT-qPCR, and identified gene A as a novel candidate of the tumor endothelium-related gene. The Cancer Genome Atlas (TCGA) datasets revealed that higher expression of gene A is associated with worse overall survival of CRC patients. Knockdown of gene A in human umbilical vein endothelial cell (HUVEC) suppressed cell proliferation and in vitro tube formation. Injection of siRNA against gene A in mice transplanted with human CRC cells suppressed formation of microvessels in the xenografted tumors. Microarray analysis revealed that knockdown of gene A in HUVEC significantly affected gene expression signatures, including cell cycle regulation and interferon signaling. Our results suggest that gene A may play an important role in the angiogenesis in CRC, and that it could be a potential therapeutic target.
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| Free Research Field |
消化器がん
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