Analysis of quasispecies of HBV / HCV genes in a single individual by a new sequencing technique

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K08995
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: University of Yamanashi
• Principal Investigator: SAKAMOTO Minoru 山梨大学, 大学院総合研究部, 准教授 (60324191)
• Co-Investigator(Kenkyū-buntansha): 榎本 信幸 山梨大学, 大学院総合研究部, 教授 (20251530)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: HBV / HCV / quasispecies

Outline of Final Research Achievements

The quasispecies state of HBV, HCV genome was analyzed by new sequencing technology.
In HCV, the drug resistance mutation against DAA is minor clone so called drug resistant associated variants: RAV becomes dominant after treatment. In HBV, quasispecies state in which deletion mutant of HBV-PreS2 region and Pre S1 / S2 initiation codon mutation are existed. It is formed by persistent infection with or without antiviral treatment and existent ratio 1% or more is associated with the disease state. Further In HCC cases, an increase in the existent ratio of mutated clone in PreS2 region is closely related to liver carcinogenesis.

Free Research Field

消化器内科学