Differentiation of human induced pluripotent stem cells to hepatocyte lineage

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K09032
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: 独立行政法人国立病院機構 下志津病院(臨床研究部)
• Principal Investigator: Tomizawa Minoru 独立行政法人国立病院機構 下志津病院(臨床研究部), 臨床研究部, その他 (90334193)
• Co-Investigator(Renkei-kenkyūsha): OHIRA Miki 千葉県がんセンター(研究所), がんゲノム研究室, 室長 (20311384)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: iPS細胞 / 肝細胞 / ブドウ糖 / アルギニン

Outline of Final Research Achievements

To promote differentiation of human induced pluripotens stem (iPS) cells to hepatocytes, oncostati M, and M50054 were added to a medium without glucose or arginine, and added with galactose and ornithine (hepatocyte differentiation inducer). The expression levels of alpha-feto protein were up-regulated in iPS cells cultured in HDI for 2 days. It was suggested that HDI promoted differentiation of iPS cells to hepatocyte lineage.

Free Research Field

消化器内科学