Analysis of the replication mechanism of hepatitis C virus in membrane vesicles containing replication complexes

2018 Fiscal Year Final Research Report

• Project/Area Number: 15K09034
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: Hideki Aizaki 国立感染症研究所, ウイルス第二部, 室長 (00333360)
• Project Period (FY): 2015-04-01 – 2019-03-31
• Keywords: HCV / プラス鎖RNAウイルス / 複製複合体 / Double membrane vesicle / SURF4

Outline of Final Research Achievements

Positive-strand RNA viruses such as hepatitis C virus (HCV), polio virus, and SARS coronavirus form membrane vesicles (Double membrane vesicles (DMV)) containing replication complexes. In this study, we analyzed the mechanism of membrane vesicle formation that is essential for HCV replication. It has been shown that the NS4B binding protein Surf4 may act to form a replication component common to positive-strand RNA viruses such as HCV and polio virus. These results indicate that Surf4 interacts with NS4B to form DMV and participate in HCV replication.

Free Research Field

ウイルス学、肝臓病学

Academic Significance and Societal Importance of the Research Achievements

C型肝炎ウイルス(HCV)、ポリオウイルス、サーズコロナウイルス等のプラス鎖RNAウイルス共通の複製膜は正常なオルガネラには存在しない特徴滴な膜構造物と考えられることから、これらの疾患の診断、治療のターゲットとなりするものと期待できる。