Clinical significance of cholesterol uptake capacity, a novel assay of high-density lipoprotein function, in coronary artery disease

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K09121
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Cardiovascular medicine
• Research Institution: Kobe University
• Principal Investigator: Tatsuro Ishida 神戸大学, 医学研究科, 特命教授 (00379413)
• Co-Investigator(Kenkyū-buntansha): 杜 隆嗣 神戸大学, 医学研究科, 特命准教授 (50379418) ; 平田 健一 神戸大学, 医学研究科, 教授 (20283880)
• Co-Investigator(Renkei-kenkyūsha): SHINKE TOSHIRO 神戸大学, 医学部附属病院, 准教授 (60379419)
• Research Collaborator: SHINOHARA MASAKAZU 神戸大学, 大学院医学研究科, 准教授 (80437483)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 高比重リポ蛋白 / コレステロール逆転送 / 動脈硬化 / 冠動脈疾患 / 脂質異常症

Outline of Final Research Achievements

We established a new method to evaluate the efficiency of HDL-induced cholesterol efflux from macrophages “cholesterol uptake capacity (CUC)” using apoB-depleted serum, fluorescently labeled cholesterol, and immobilized anti-apolipoprotein A1 antibody. When we measured CUC in patients with coronary artery disease, CUC declined with myeloperoxidase-mediated oxidation of HDL, and correlated inversely with requirement of revascularization. Multivariable analysis adjusted for traditional coronary risk factors including HDL-C showed that only CUC remained significant. Furthermore, CUC was lower in patients with lipidic plaque than that in those without lipidic plaque, and inversely associated with the lipid index in these patients. CUC was also inversely associated with Macrophage grade. CUC is an alternative measure of HDL functionality and may be a substitute for cholesterol efflux capacity and applicable for assessing CVD risk.

Free Research Field

循環器内科