Generator mechanism and dynamics of functional brain networks of epileptic slow activities

2017 Fiscal Year Annual Research Report

• Project/Area Number: 15K09351
• Research Institution: Kyoto University
• Principal Investigator: 井内 盛遠 京都大学, 医学研究科, 客員研究員 (30532600)
• Co-Investigator(Kenkyū-buntansha): 松本 理器 京都大学, 医学研究科, 准教授 (00378754) ; 麻生 俊彦 京都大学, 医学研究科, 特定講師 (50397543) ; 國枝 武治 愛媛大学, 医学系研究科, 教授 (60609931) ; 池田 昭夫 京都大学, 医学研究科, 特定教授 (90212761)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: てんかん / 低周波活動 / 脳内ネットワーク / 機能的MRI

Outline of Annual Research Achievements

1) てんかん焦点における広域周波数帯域脳波解析を用いた低周波活動の記録解析：多施設からの難治部分てんかん患者を対象に共通の方法を用いて広域周波数帯域皮質脳波解析を行った結果、てんかん発作発現に伴う低周波活動である発作時DC電位（グリアの関与が示唆）が、高周波律動（HFO：ニューロンの高頻度発火、介在ニューロンの活動を反映）に対して早期に出現することを明らかにした（国内学会発表）。既報告に合致した結果であり、発作発現時にグリアとニューロンが密接に関連しつつ異なる役割を担う可能性が示唆された。多施設における解析の過程で得られた知見をもとに広域周波数帯域脳波の記録・解析標準化案を作成した（論文発表）。
頭蓋内電極留置期間中に、頭皮上に少数の電極を貼付して睡眠段階を同定し得た患者において、覚醒・睡眠段階で分類した皮質脳波を用いた異周波数間結合の解析を継続した。高周波数帯域の瞬時振幅と低周波数帯域の瞬時位相の相関解析（phase-amplitude coupling）を行い、徐波睡眠期に低周波と高周波数帯域の異周波数間結合が強まること、脳葉、てんかん原性の有無によって結合の程度、睡眠段階による結合動態が異なることを明らかにした（国際学会発表、論文準備中）。
2) 機能的MRI（fMRI）を用いた記録解析：視床下部過誤腫におけるてんかん患者を対象にてんかん外科手術前後の安静時fMRIの記録・解析を行った。てんかん焦点である視床下部過誤腫と大脳皮質間の機能的結合は術後に減弱する一方で、他の機能的ネットワークについては有意な相関を示すものが術後に増加しており、手術によるてんかん発作の抑制、てんかん性脳症の改善との関連性が推察された（国内学会発表）。

Research Products

• [Journal Article] High frequency activity overriding cortico-cortical evoked potentials reflects altered excitability in the human epileptic focus. (2017)
  • Author(s): Kobayashi K, Matsumoto R, Matsuhashi M, Usami K, Shimotake A, Kunieda T, Kikuchi T, Yoshida K, Mikuni N, Miyamoto S, Fukuyama H, Takahashi R, Ikeda A
  • Journal Title: Clinical Neurophysiology. Volume: 128 Pages: 1673-1681
  • DOI: 10.1016/j.clinph.2017.06.249.
  • Peer Reviewed / Open Access
• [Journal Article] Phasic REM Transiently Approaches Wakefulness in the Human Cortex―A Single-Pulse Electrical Stimulation Study. (2017)
  • Author(s): Usami K, Matsumoto R, Kobayashi K, Hitomi T, Matsuhashi M, Shimotake A, Kikuchi T, Yoshida K, Kunieda T, Mikuni N, Miyamoto S, Takahashi R, Ikeda A
  • Journal Title: Sleep. Volume: 40 Pages: -
  • DOI: 10.1093/sleep/zsx077.
  • Peer Reviewed
• [Journal Article] Clinical impact of intraoperative CCEP monitoring in evaluating the dorsal language white matter pathway. (2017)
  • Author(s): Yamao Y, Suzuki K, Kunieda T, Matsumoto R, Arakawa Y, Nakae T, Nishida S, Inano R, Shibata S, Shimotake A, Kikuchi T, Sawamoto N, Mikuni N, Ikeda A, Fukuyama H, Miyamoto S
  • Journal Title: Human Brain Mapping. Volume: 38 Pages: 1977-1991
  • DOI: doi: 10.1002/hbm.23498
  • Peer Reviewed
• [Journal Article] Frontal Fibers Connecting the Superior Frontal Gyrus to Broca Area: A Corticocortical Evoked Potential Study. (2017)
  • Author(s): Ookawa S, Enatsu R, Kanno A, Ochi S, Akiyama Y, Kobayashi T, Yamao Y, Kikuchi T, Matsumoto R, Kunieda T, Mikuni N
  • Journal Title: World Neurosurgery. Volume: 107 Pages: 239-248
  • DOI: 10.1016/j.wneu.2017.07.166.
  • Peer Reviewed
• [Journal Article] Magnetoencephalography with temporal spread imaging to visualize propagation of epileptic activity. (2017)
  • Author(s): Shibata S, Matsuhashi M, Kunieda T, Yamao Y, Inano R, Kikuchi T, Imamura H, Takaya S, Matsumoto R, Ikeda A, Takahashi R, Mima T, Fukuyama H, Mikuni N, Miyamoto S
  • Journal Title: Clinical Neurophysiology Volume: 128 Pages: 734-743
  • DOI: 10.1016/j.clinph.2017.01.010.
  • Peer Reviewed
• [Journal Article] Neural pattern similarity between contra- and ipsilateral movements in high-frequency band of human electrocorticograms. (2017)
  • Author(s): Fujiwara Y, Matsumoto R, Nakae T, Usami K, Matsuhashi M, Kikuchi T, Yoshida K, Kunieda T, Miyamoto S, Mima T, Ikeda A, Osu R.
  • Journal Title: Neuroimage Volume: 15 Pages: 302-313
  • DOI: doi: 10.1016/j.neuroimage.
  • Peer Reviewed / Open Access
• [Journal Article] Single pulse electrical stimulation to probe functional and pathological connectivity in epilepsy (2017)
  • Author(s): Matsumoto R, Kunieda T, Nair D.
  • Journal Title: Seizure Volume: 44 Pages: 27-36
  • DOI: 10.1016/j.seizure.2016.11.003.
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Standards for data acquisition and software-based analysis of in vivo electroencephalography recordings from animals: report from the ILAE-AES joint translational task force (2017)
  • Author(s): Moyer JT, Gnatkovsky V, Ono T, Otahal J, Wagenaar J, William C. Stacey W, Noebels J, Ikeda A, Staley K, de Curtis M, Litt B, Galanopoulou AS
  • Journal Title: Epilepsia Volume: 58 Pages: 53-67
  • DOI: 10.1111/epi.13909
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] How to record high frequency oscillations in epilepsy: a practical guideline (2017)
  • Author(s): Zijlmans M, Worrell G, Duempelmann M, Stieglitz T, Barborica A, Heers M, Ikeda A, Usui N, Le Van Quyen M
  • Journal Title: Epilepsia Volume: 58 Pages: 1305-1315
  • DOI: doi: 10.1111/epi.13814
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] We could predict good responders to vagus nerve stimulation: A surrogate marker by slow cortical potential shift. (2017)
  • Author(s): Bayasgalan B, Matsuhashi M, Fumuro T, Nohira H, Nakano N, Iida K, Katagiri M, Shimotake A, Matsumoto R, Kikuchi T, Kunieda T, Kato A, Takahashi R, Ikeda A
  • Journal Title: Clinical Neurophysiology Volume: 128 Pages: 1583-1589
  • DOI: 10.1016/j.clinph.2017.05.019
  • Peer Reviewed
• [Journal Article] Inhibition of inwardly rectifying potassium (Kir) 4.1 channels facilitates brain-derived neurotrophic factor (BDNF) expression in astrocytes. (2017)
  • Author(s): M. Kinboshi T. Mukai Y. Nagao Y. Matsuba Y. Tsuji S. Tanaka K. Tokudome S. Shimizu H. Ito, A, Ikeda A. Inanobe Y. Kurachi S. Inoue Y. Ohno:
  • Journal Title: Frontiers in Molecular Neuroscience Volume: 10 Pages: -
  • DOI: 10.3389/fnmol.2017.00408
  • Peer Reviewed / Open Access
• [Journal Article] Lateralization of intrinsic frontoparietal network connectivity and symptoms in schizophrenia (2017)
  • Author(s): Son S, Miyata J, Mori Y, Isobe M, Urayama SI, Aso T, Fukuyama H, Murai T, Takahashi H
  • Journal Title: Psychiatry Research: Neuroimaging Volume: 260 Pages: 23-28
  • DOI: 10.1016/j.pscychresns.2016.12.007
  • Peer Reviewed
• [Journal Article] Wide band EEGの有用性 (slow) (2017)
  • Author(s): 井内盛遠、中谷光良、池田昭夫
  • Journal Title: 臨床神経生理学 Volume: 45 Pages: 520-524
  • DOI: 10.11422/jscn.45.520
• [Journal Article] 難治部分てんかん患者の焦点検索における、発作時DC電位・HFOの記録および解析の標準化案 (2017)
  • Author(s): 中谷光良、井内盛遠、大封昌子、十川純平、村井智彦、橋本聡華、稲次基希、白水洋史、金澤恭子、渡辺裕貴、臼井直敬、井上有史、前原健寿、池田昭夫
  • Journal Title: てんかん研究 Volume: 35 Pages: 3-13
  • DOI: 10.3805/jjes.35.3
  • Peer Reviewed
• [Journal Article] CCEPのオフライン解析と解釈 特集「CCEPのskills workshop:clinical practice parameter(臨床実践 指標)を目指して」 (2017)
  • Author(s): 小林 勝哉、松本 理器、松橋 眞生、中江 卓郎、宇佐美 清英、山尾 幸宏、菊池 隆幸、吉田 和 道、國枝 武治、池田 昭夫
  • Journal Title: 臨床神経生理学 Volume: 45 Pages: 91-101
  • DOI: 10.11422/jscn.45.91
  • Peer Reviewed
• [Presentation] How do we delineate the source of wide-band EEG data: Epileptic DC shifts and HFO? (2017)
  • Author(s): Ikeda A, Matsumoto R, Kobayashi K, Kunieda T
  • Organizer: Biomagnetic Sendai 2017
  • Int'l Joint Research / Invited
• [Presentation] Can infraslow and DC shift improve epilepsy treatment ? (2017)
  • Author(s): Ikeda A
  • Organizer: Beijing Epilepsy and Neurophysiology Summit (BENS2017)
  • Int'l Joint Research / Invited
• [Presentation] Neural basis of altered consciousness during sleep: insights from effective connectivity and induced high gamma activities (2017)
  • Author(s): Matsumoto R, Usami K, Kikuchi T, Yoshida K, Ikeda A
  • Organizer: Neural Oscillation Conference 2017
  • Int'l Joint Research / Invited
• [Presentation] Epileptic DC shift and its epileptogenic mechanism (2017)
  • Author(s): Inouchi M, Daifu M, Nakatani M, Ikeda A
  • Organizer: The 51th Congress of the Japanese Epilepsy Society annual meeting.
  • Invited
• [Presentation] てんかん発作時の wide!band EEG(発作時 DC 電位・HFO の意義 (2017)
  • Author(s): 井内盛遠
  • Organizer: The 51th Congress of the Japanese Epilepsy Society annual meeting.
  • Invited
• [Presentation] Cortico-cortical evoked potentials & soectral responses to probe connectivity and cortical excitability. (2017)
  • Author(s): Matsumoto R
  • Organizer: 51st Annual Congress of the Japan Epilepsy Societyannual meeting.
  • Invited
• [Presentation] Novel surrogate biomarkers of epileptogenicity using wideband ECoG analyses. (2017)
  • Author(s): Matsumoto R, Kobayashi K, Kikuchi T, Yoshida K, Kunieda T, Miyamoto S, Ikeda A
  • Organizer: The 76th Annual Meeting of the Japan Neurosurgery Society
  • Invited
• [Presentation] Introduction. Dual stream model of language - its function, connectivity and plasticity: insights from clinical neuroscience (2017)
  • Author(s): Matsumoto R
  • Organizer: The 40th Annual Meeting of the Japan Neuroscience Society (Neuroscience 2017)
  • Invited
• [Presentation] Glial dysfunction and intractable epilepsy-from basic to clinical studies-Glia, DC shifts/red slow, and epilepsy (難治てんかん焦点におけるグリアの役割:臨床神経生理学からのアプローチ) (2017)
  • Author(s): Ikeda A
  • Organizer: Annual congress of 60th Japanese Society for Neurochemistry
  • Invited
• [Presentation] Neuron, glia and epilepsy:is it a paradigm shift? (2017)
  • Author(s): 池田昭夫
  • Organizer: Annual congress of 51th Japanese Epilepsy Society
  • Invited
• [Presentation] 皮質脳波と高次脳機能ネットワーク (2017)
  • Author(s): 松本理器、下竹昭寛，菊池隆幸，國枝武治、池田昭夫
  • Organizer: 第47回日本臨床神経生理学会
  • Invited
• [Presentation] Spatio-temporal dynamics of interaction between slow and fast waves in humans during sleep (2017)
  • Author(s): Togawa J, Inouchi M, Matsumoto R, Matsuhashi M, Kobayashi K, Usami K, Hitomi T, Nakae T, Kikuchi T, Yoshida K, Kunieda T, Miyamoto S, Takahashi R, Ikeda A
  • Organizer: The 71st American Epilepsy Society Annual meeting 2017
  • Int'l Joint Research
• [Presentation] Co-occurrence of slow and high frequency oscillations (HFOs) in invasively recorded, interictal state in epilepsy patients: Is it a 'Red slow'? (2017)
  • Author(s): Daifu M, Inouchi M, Matsuhashi M, Kobayashi K, Shimotake A, Hitomi T, Yoshida K, Kunieda T, Matsumoto R, Takahashi R, Ikeda A
  • Organizer: The XXXⅢ World Congress of Neurology
  • Int'l Joint Research
• [Presentation] Electric cortical stimulation inhibits interictal epileptiform discharge and related high frequency activity in human epileptic focus. (2017)
  • Author(s): Nakatani M, Matsumoto R, Kobayashi K, Hitomi T, Inouchi M, Matsuhashi M, Kinoshita M, Kikuchi T, Yoshida K, Kunieda T, Takahashi R, Hattori N and Ikeda A
  • Organizer: The 71st American Epilepsy Society Annual meeting 2017
  • Int'l Joint Research
• [Presentation] Safety of single-pulse electrical stimulation for cortico-cortical evoked potentials in epileptic human cerebral cortex (2017)
  • Author(s): Kobayashi K, Matsumoto R, Usami K, Matsuhashi M, Shimotake A, Nakae T, Takeyama H, Kikuchi T, Yoshida K, Kunieda T, Takahashi R, Ikeda A
  • Organizer: The XXXⅢ World Congress of Neurology
  • Int'l Joint Research
• [Presentation] Invasive 3D source localization by wide-band electroencephalography findings (2017)
  • Author(s): Murai T, Matsuhashi M, Hitomi T, Kobayashi K, Shimotake A, Inouchi M, Kikuchi T, Yoshida K, Kunieda T, Matsumoto R, Takahashi R, Ikeda A
  • Organizer: The XXXⅢ World Congress of Neurology
  • Int'l Joint Research
• [Presentation] Inhibitory effects of electric cortical stimulation on interictal epileptiform discharges in human epileptic focus (2017)
  • Author(s): Nakatani M, Matsumoto R, Kobayashi K, Hitomi T, Inouchi M, Matsuhashi M, Kinoshita M, Kikuchi T, Yoshida K, Kunieda T, Takahashi R, Hattori N and Ikeda A
  • Organizer: The XXXⅢ World Congress of Neurology
  • Int'l Joint Research
• [Presentation] Proposal of standard recording and analysis intracranial ictal DC shifts and HFOs. (2017)
  • Author(s): Nakatani M, Inouchi M, Togawa J, Murai T, Kobayashi K, Hitomi T, Hashimoto S, Inani M, Shiroku H, Kanazawa K, Watanabe Y, Usui N, Inoue Y, Maehara T and Ikeda A
  • Organizer: The 51th Congress of the Japanese Epilepsy Society annual meeting.
• [Presentation] Inhibitory effects of electric cortical stimulation on interictal epileptiform discharges in human epileptic focus. (2017)
  • Author(s): Nakatani M, Matsumoto R, Kobayashi K, Hitomi T, Inouchi M, Matsuhashi M, Kinoshita M, Kikuchi T, Yoshida K, Kunieda T, Takahashi R, Hattori N and Ikeda A
  • Organizer: 第40回日本神経科学学術大会
• [Presentation] Increase of resting state functional connectivity after stereotactic radiofrequency thermocoagulation in the patients of hypothalamic hamartoma. (2017)
  • Author(s): Jumpei Togawa, Yosuke Ito, Toshihiko Aso, Riki Matsumoto, Morito Inouchi, Hiroshi Shirozu, Hiroshi Masuda, Ryosuke Takahashi, Masafumi Fukuda, Akio Ikeda, Shigeki Kameyama
  • Organizer: The 51th Congress of the Japanese Epilepsy Society annual meeting.
• [Presentation] Source localization of seizure focus by wide-band stereoelectroencephalography (2017)
  • Author(s): Murai T, Matsuhashi M,Hitomi T, Toho M, Kobayashi K, Shimotake A, Inouchi M, Kikuchi K, Yoshida K, Kunieda R, Matsumoto R, Kanda R, Takahashi R, Ikeda A
  • Organizer: 第51回日本てんかん学会学術集会
• [Presentation] Evidence for the engagement of cortico-cortical and cortico-subcortical networks in generation of epileptic spasms: a combined case study using CCEP and EEG-fMRI (2017)
  • Author(s): Inoue T, Kobayashi K, Matsumoto R, Inouchi M, Togo M, Togawa J, Usami K, Shimotake A, Matsuhashi M, Kikuchi T, Yoshida K, Kawawaki H, Kunieda T, Takahashi R, Ikeda A.
  • Organizer: 第51回日本てんかん学会学術集会
• [Presentation] Clinical diversity of DC shift on scalp electroencephalography (2017)
  • Author(s): Murai T, Hitomi T, Kawamura Y, Kobayashi K, Shimotake A, Matsuhashi M, Matsumoto R, Kanda R, Takahashi R, Ikeda A
  • Organizer: 第49回日本臨床神経生理学会学術大会
• [Presentation] Direct current shift on scalp electroencephalography recorded in time constant 2 sec (2017)
  • Author(s): Murai T, Hitomi T, Kawamura Y, Matsumoto R, Kanda R, Takahashi R, Ikeda A
  • Organizer: 第51回日本てんかん学会学術集会
• [Presentation] The human entorhinal cortex is connected with the broad neocortical regions through the hippocampalthalamo-cortical pathway possible as the memory network: Results from electrical tract tracing and neuropsychologicalassessment (2017)
  • Author(s): Takeyama H, Matsumoto R, Nakae T, Kobayashi K, Usami K, Shimotake A, Kikuchi T, Yoshida K, Kunieda T, Miyamoto S, Takahashi R Ikeda A
  • Organizer: 第40回日本神経科学大会
• [Presentation] Wide-band EEG analysis of epileptic seizures during pilocarpine induced status epilepticus in rat (2017)
  • Author(s): Sato K, Kinboshi M, I. Higor A, Mukai T, Matsuhashi M, Ikeda A, Ohno Y.
  • Organizer: 第51回てんかん総会学術集会
• [Presentation] Dysfunction of Kir4.1 channels facilitates BDNF production in astrocytes (2017)
  • Author(s): Kinboshi M, Mukai T, Nagao Y, Shimizu S, Ito H, Ikeda A, Ohno Y.
  • Organizer: 第 51 回日本てんかん学会学術集会
• [Presentation] 難治部分てんかん患者での単発皮質電気刺激の発作誘発率に関する安全性：皮質皮質間誘発電位計測時の検討 (2017)
  • Author(s): 小林勝哉, 松本理器, 宇佐美清英, 松橋眞生, 下竹昭寛, 中江卓郎, 武山博文, 菊池隆幸, 吉田和道, 國枝武治, 高橋良輔 and 池田昭夫
  • Organizer: 第47回日本臨床神経生理学会学術大会
• [Presentation] 発作時直流緩電位（Ictal DC shifts）の時定数10秒と2秒の記録・表示における相違：臨床的意義の検討 (2017)
  • Author(s): 梶川駿介, 小林勝哉, 中谷光良, 村井智彦, 金澤恭子, 松橋眞生, 下竹昭寛, 人見健文, 松本理器, 高橋良輔 and 池田昭夫
  • Organizer: 日本てんかん学会第51回年次集会
• [Presentation] 難治性部分てんかん患者での発作時高周波律動の時間的周波数変化と臨床相関 (2017)
  • Author(s): 藤井大樹, 小林勝哉, 下竹昭寛, 金澤恭子, 菊池隆幸, 吉田和道, 松本理器, 國枝武治, 髙橋良輔 and 池田昭夫
  • Organizer: 第47回日本臨床神経生理学会学術大会
• [Presentation] 質電気刺激による行為関連ネットワークマッピング (2017)
  • Author(s): 下竹昭寛,松本理器,小林勝哉,國枝武治,三國信啓,宮本亨,高橋良輔,池田昭夫
  • Organizer: 第47回日本臨床神経生理学会
• [Book] Invasive Studies of the Human Epileptic Brain: Principles and Practice of Invasive Brain Recordings and Stimulation in Epilepsy (2018)
  • Author(s): Ikeda A
  • Total Pages: in press
  • Publisher: Oxford University Press (London)
• [Book] Invasive Studies of the Human Epileptic Brain: Principles and Practice of Invasive Brain Recordings and Stimulation in Epilepsy (2018)
  • Author(s): Matsumoto R, Kunieda T
  • Total Pages: in press
  • Publisher: Oxford University Press (London)
• [Book] 脳神経外科 - (4) Wide-band EEG を用いた焦点診断 - グリアとニューロン両者からのアプローチ (2018)
  • Author(s): 中谷光良、井内盛遠、前田健寿、池田昭夫
  • Total Pages: -
  • Publisher: 医学書院
• [Book] ガイトン生理学 - 脳の活動状態：睡眠、脳波、てんかん、精神病、認知症 (2018)
  • Author(s): 十川純平、音成秀一郎、十河正弥、池田 昭夫
  • Total Pages: 684-693
  • Publisher: エルセビア・ジャパン
• [Book] 診断と治療特集 一般臨床医に必要なてんかんの基礎知識とトピックス，鈴木則宏編 (2017)
  • Author(s): 井内盛遠，松本理器
  • Total Pages: 837-842
  • Publisher: 診断と治療社
• [Book] Clinical Neuroscience 37(7) - 直流脳波(DC電位） (2017)
  • Author(s): 村井智彦，井内盛遠，池田昭夫
  • Total Pages: 822-825
  • Publisher: 中外医学社
• [Book] Clinical Neuroscience 35(7) - 硬膜下電極記録 (2017)
  • Author(s): 中谷光良，小林勝哉，松本理器
  • Total Pages: 826-829
  • Publisher: 中外医学社