The intrinsic roles of human PAI-1 in adipocyte differentiation and function with using patient-derived iPS cells.

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K09411
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Metabolomics
• Research Institution: Hamamatsu University School of Medicine
• Principal Investigator: Sano Hideto 浜松医科大学, 医学部, 助教 (80623842)
• Co-Investigator(Kenkyū-buntansha): 浦野 哲盟 浜松医科大学, 医学部, 教授 (50193967) ; 鈴木 優子 浜松医科大学, 医学部, 准教授 (20345812) ; 田中 宏樹 浜松医科大学, 医学部, 助教 (50456563)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: PAI-1 / adipocytes / endothelial cells / iPS cells

Outline of Final Research Achievements

We have generated iPS cells (PAI-1 iPS) from PAI-1 deficient patients, then differentiated them into mature cells, and analyzed the intrinsic function of human PAI-1. We have attempted adipocyte differentiation from PAI-1 iPS. Mesenchymal stem cell (MSC) - like cells could be detected, but we are still inquiring into efficient condition of adipocyte differentiation. On the other hand, in the gene expression analysis of iPS cell-derived endothelial cells (iPS-ECs) which are already established, we observed the expression of hormone-like protein genes that involved in adipocyte differentiation such as IGFBP 3 and 5. Currently, this iPS-ECs are co-cultured with the above MSC-like or adipocyte precursor cell line such as 3T3-L1 cells, and confirmed PAI-1 functions and the effects on adipocyte differentiation.

Free Research Field

細胞生理学