Elucidation of the role of kindlin in the functional expression of beta3 integrins and investigation of the kindlin-related new molecules

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K09463
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Hematology
• Research Institution: National Cardiovascular Center Research Institute
• Principal Investigator: Honda Shigenori 国立研究開発法人国立循環器病研究センター, 研究所, 室長 (00303959)
• Co-Investigator(Kenkyū-buntansha): 宮田 敏行 国立研究開発法人国立循環器病研究センター, 病院, 非常勤研究員 (90183970)
• Co-Investigator(Renkei-kenkyūsha): Tomiyama Yoshiaki 大阪大学, 医学研究科, 准教授 (80252667)
• Research Collaborator: Ikejima Hiroko 国立循環器病研究センター, 研究所, 研究補助者
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: インテグリン

Outline of Final Research Achievements

β3 integrins are heterodimeric adhesion receptors and members of the integrin superfamily consisted of αIIbβ3 and αVβ3. αIIbβ3, a major integrin expressed on platelets, is critical for platelet aggregation mediated by bindings of fibrinogen and von Willebrand factor. αVβ3 (also called vitronectin receptor), an integrin widely expressed on several cell types including blood vessel tissue constitutive cells and tumor cells, is involved in diverse vascular disease states, tumor proliferation and metastasis.
Kindlins function as an integrin activator that interacts with intracellular proteins such as integrin-linked kinase (ILK). We have reported that ILK is associated with integrin activation. In this study, we investigated the role of each domain of Kindlin-3 in αIIbβ３ activation, and it was suggested that each subdomain could have an important role in αIIbβ3 activation.

Free Research Field

血栓止血学