Longitudinal analyses of genetic alterations in malignant lymphoma using cell-free DNA: detection of refractory disease related abnormalities and application possibility in clinical trials.

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K09473
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Hematology
• Research Institution: Fujita Health University
• Principal Investigator: Tomita Akihiro 藤田保健衛生大学, 医学部, 准教授 (80378215)
• Co-Investigator(Renkei-kenkyūsha): YOSHIDA Kenichi 京都大学, 医学部・腫瘍生物学講座 (50738226)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 悪性リンパ腫 / 網羅的遺伝子変異解析 / 末梢血無細胞遊離DNA / リキッドバイオプシー / 血管内大細胞型B細胞リンパ腫

Outline of Final Research Achievements

Accumulation of genetic mutations is critical for lymphomatogenesis, disease progression and sensitivity to targeting therapeutics in malignant lymphoma (ML). In this analysis, peripheral blood cell-free DNA (cfDNA) was longitudinally analyzed to reveal correlation with clinical features and to detect tumor specific genetic alterations. Especially in intravascular large B-cell lymphoma, those tumor cells are hardly obtained from biopsy sample, cfDNA was an alternative tumor DNA source to detect specific genetic alterations in comprehensive genetic analyses. Genetic analyses using cfDNA may become an alternative powerful strategy to detect tumor specific genetic abnormalities not only for diagnosis but also longitudinal analyses to predict sensitivity to molecular targeting drugs, even in patients whose tumor cells cannot be obtained easily during their clinical courses.

Free Research Field

血液腫瘍学