Molecular analysis of variety of genomic rearrangements and chimeric genes in tumorigenesis.

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K09486
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Hematology
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: TAKI TOMOHIKO 京都府立医科大学, 医学(系)研究科(研究院), 講師 (50322053)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: がん / ゲノム / キメラ遺伝子 / conjoined gene / エクソン / イントロン

Outline of Final Research Achievements

We identified the conjoined genes and exonization of intron sequence in chimeric transcripts in hematologic malignancies. Conjoined gene is a chimeric transcript created with the genes adjacent each other, and the novel eleven conjoined genes were identified in a diffuse large B-cell lymphoma cell line in this study. Genome array analysis did not detect any copy number alterations in the regions of conjoined genes, suggesting the formation of fusion genes in transcriptional level. On the other hand, in chimeric transcripts created by chromosome translocation, some intronic sequences were inserted into chimeric transcripts occasionally, suggesting the exonization of intron by gene rearrangement in chromosomal translocations.

Free Research Field

血液・腫瘍学、分子遺伝学