2017 Fiscal Year Final Research Report
NETs treated monocytes in immune system
| Project/Area Number |
15K09545
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Collagenous pathology/Allergology
|
|---|
| Research Institution | Hokkaido University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2015-04-01 – 2018-03-31
|
| Keywords | 自然免疫 |
|---|
| Outline of Final Research Achievements |
Human healthy CD14+ cells treated with neutrophil extra cellular traps (NETs) could differentiate into CD14+CD1a-CD11c+HLA-DR+ cells assessed by flow cytometry. Expression levels of type-I interferon mRNA including interferon-alpha and interferon-beta were increased in these cells quantified by real-time PCR. On the other hand, expression levels of interferon-gamma, type-II interferon, mRNA were not significant increased. These expression levels could not be confirmed at the protein levels by western blot or flow cytometry because of its low expression.
|
| Free Research Field |
臨床免疫学
|
