Analysis of microRNAs related to the development of infantile atopic dermatitis

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K09638
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pediatrics
• Research Institution: Chiba University
• Principal Investigator: Inoue Yuzaburo 千葉大学, 大学院医学研究院, 特任講師 (00456063)
• Co-Investigator(Renkei-kenkyūsha): SHIMOJO Naoki 千葉大学, 大学院医学研究院, 教授 (40221303) ; YAMAIDE Fumiya 千葉大学, 医学部附属病院, 助教 (50636199) ; NAKANO Taiji 千葉大学, 医学部附属病院, 助教 (90708306)
• Research Collaborator: Dissanayake Eishika
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: アトピー性皮膚炎 / microRNA / 表皮角化細胞 / コホート研究

Outline of Final Research Achievements

We found that the microRNA, hsa-miR-144-3p (miR-144), is highly expressed in the umbilical cord serum of infants who developed AD at 1 year of age. This change was not evident in maternal serum or at 1 year of age. The miR-144 levels did not correspond to the serum IgE levels at 1 year of age as well. Mir-144 targets ABCA1 and led to a decrease in ABCA1 levels and cholesterol accumulation in miR-144-transfected human primary epithelial keratinocytes. In response to house dust mite (HDM) stimulation, miR-144-transfected keratinocytes showed an increase in the translocation of the NF-κB p65 subunit. HDM stimulation also led to an increase in the mRNA levels of human beta defensin-2, which has been shown to promote mast cell degranulation, following miR-144 transfection of keratinocytes. These findings suggest that high levels of miR-144, compounded via NF-kB activation maybe an early atopy-independent trigger for the development of AD at 1 year of age.

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