Research on Pathogenesis of Acute Rupture of Chordae Tendinae of the Mitral Valve in Infants: Possible involvement of maternally derived anti-SSA antibody

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K09705
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pediatrics
• Research Institution: National Cardiovascular Center Research Institute
• Principal Investigator: Shiraishi Isao 国立研究開発法人国立循環器病研究センター, 病院, 部長 (80295659)
• Co-Investigator(Renkei-kenkyūsha): SHIRAI Manabu 国立研究開発法人国立循環器病研究センター, 創薬オミックス解析センター, 室長 (70294121)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 僧帽弁腱索断裂 / 抗SSA抗体 / メタゲノム解析 / RNAトランスクリプトーム解析 / 川崎病

Outline of Final Research Achievements

In order to elucidate the pathogenesis and pathophysiology of the mitral chordal rupture in infants, metagenome analysis was performed using a fresh mitral valve tissue of an infant with mitral chordal rupture. RNA transcriptome analysis was also performed using formalin fixed paraformaldehyde embedded (FFPE) mitral valve tissues taken from the 4 patients and 2 controls. So far, specific viral or bacterial genome has not been detected in metagenome analysis. Specific RNA up regulation and down regulation have not been identified including pathways concerning anti-SSA antibody, either.
To be able to obtain convincing results and elucidate pathogenesis and pathophysiology of the disease, metagenome and RNA transcriptome analysis with more samples are necessary.

Free Research Field

小児科学