Subzero lung preservation in a variable magnetic field.using anti-freeze protein

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K10021
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General surgery
• Research Institution: Kanazawa University
• Principal Investigator: Matsumoto Isao 金沢大学, 医学系, 准教授 (80361989)
• Co-Investigator(Kenkyū-buntansha): 田村 昌也 金沢大学, 大学病院, 助教 (10397185) ; 高田 宗尚 金沢大学, 医学系, 協力研究員 (20459514) ; 加藤 寛城 金沢大学, 附属病院, 助教 (20733843)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 肺保存 / 不凍物質 / 微弱地場 / サブゼロ

Outline of Final Research Achievements

The effectiveness of subzero lung preservation in a variable magnetic field using CAS with anti-freeze protein was examined.
We prepared a lung allograft model in rats. After reperfusion for 2 hours following 24-hour preservation, we evaluated the energy charge and reperfusion injury values of the transplanted lung in four groups. Group A: without preservation, Group B: at 4°C in a normal refrigerator, Group C: at -2°C in a CAS, Group D: 60 mg/ml of AFP to the preservation solution used for Group C. The outcome of Group C was better than that of Group B and equivalent to that of Group A and Group D. CAS was effective and adding AFP resulted in only a small added effectiveness. In addition, the effectiveness of anti-freeze proteins (AFP and AFGP) was examined in the same model as Group B. Anti-freeze proteins, in particular AFGP, are relatively effective. Furthermore, a separate experiment demonstrated that CAS with AFP was not significantly effective for -10°C lung cryopreservation.

Free Research Field

呼吸器外科