2017 Fiscal Year Final Research Report
Integrated analysis on the metastasis of colorectal cancer using next generation sequence
| Project/Area Number |
15K10152
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Digestive surgery
|
|---|
| Research Institution | Nihon University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
檜垣 時夫 日本大学, 医学部, 助教 (70246883)
|
|---|
| Project Period (FY) |
2015-04-01 – 2018-03-31
|
| Keywords | 統合解析 / ネットワーク解析 / DNAメチル化 / 大腸癌 / 転移 / 次世代シーケンス |
|---|
| Outline of Final Research Achievements |
High-throughput biological technologies have been producing a vast amount of multi-omics data regarding cancer genomics. We performed integrated analysis using gene expression and DNA methylation profiles of 116 colorectal cancer samples. First, based on gene expression values, colorectal cancer samples were divided into three clusters (Cluster-1, -2, and -3). The 5-year overall survival rates were 74.8%, 29.2%, and 29.4% in Cluster-1, -2, and -3, respectively. Second, each cluster was divided into two subgroups based on methylation status, and the 5-year overall survival rate of Cluster-1H (36.8%) was significantly shorter than that of Cluster-1L (96.1%). Third, network-based analysis using expression and methylation profiles demonstrated that the PTX3 gene was highly methylated in Cluster-1H. These combined data indicate that integrated analysis can identify disease characteristics that would be missed using single comprehensive analysis.
|
| Free Research Field |
消化器外科学
|
