Development of human iPS cell-derived multidisciplinary thick viable organized tissues aiming for myocardial regenerative therapy

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K10210
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Cardiovascular surgery
• Research Institution: Kyoto University
• Principal Investigator: Nakata Tomohiro 京都大学, 医学研究科, 助教 (00559685)
• Co-Investigator(Kenkyū-buntansha): 池田 義 京都大学, 医学研究科, 准教授 (40281092) ; 田畑 泰彦 京都大学, ウイルス・再生医科学研究所, 教授 (50211371)
• Co-Investigator(Renkei-kenkyūsha): YAMASHITA JUN 京都大学, その他部局等, 教授 (50335288)
• Research Collaborator: MASUMOTO HIDETOSHI 京都大学, 大学院医学研究科, 特定助教 (70645754)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: iPS細胞 / バイオエンジニアリング / 心臓再生

Outline of Final Research Achievements

We established a technology to generate human iPS cell-derived thick viable cardiac tissues. We validated the therapeutic potential of the tissues for porcine sub-acute myocardial infarction model, rat chronic myocardial infarction model and hamster dilated cardiomyopathy modei. Histological studies revealed the therapeutic mechanisms including the attenuation of fibrosis, angiogenesis, the attenuation of apoptosis and cardiomyocyte hypertrophy. We also established the method to generate cardiac tissue sheets and thick viable tissues from clinical-graded stock human iPS cells from Center for iPS cell Research and Application, Kyoto University. There results including the validation of the therapeutic effects of the bioengineeded tissue and the therapeutic mechanism would serve as a basis for iPS cell-based cardiac regenerative therapy.

Free Research Field

心臓血管外科学