Development of a new vascular regenerative therapy for severe limb ischemia by the transplantation of human iPS cell-derived vascular cells

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K10241
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Cardiovascular surgery
• Research Institution: Kyoto University
• Principal Investigator: SAKATA RYUZO 京都大学, 医学研究科, 名誉教授 (20325781)
• Co-Investigator(Kenkyū-buntansha): 池田 義 京都大学, 医学研究科, 准教授 (40281092) ; 山崎 和裕 京都大学, 医学研究科, 講師 (50464227)
• Co-Investigator(Renkei-kenkyūsha): YAMASHITA JUN 京都大学, その他部局等, 教授 (50335288)
• Research Collaborator: MASUMOTO HIDETOSHI 京都大学, 大学院医学研究科, 特定助教 (70645754)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 末梢動脈外科学 / iPS細胞 / 血管新生 / 血管内皮細胞

Outline of Final Research Achievements

The transplantation of human iPS cell-derived endothelial cells and vascular mural cells with gelatin sponges revealed a significant improvement of blood flow and a higher vascular density in the ischemic lindlimb. Ku-80 positive human cells are observed in the newly-formed vasculature indicating the contribution of transplanted human cells toward the vascular regeneration of the host. Furthermore, the transplantation induced the angiogenesis mediated by the up-regulation of paracrine factors. There results would serve as a basis toward the development of a new vascular regenerative therapy for a severe limb ischemia in the future.

Free Research Field

心臓血管外科学