Investigation of barrier function of endothelial cells derived from moyamoya disease-specific iPS cells

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K10286
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Neurosurgery
• Research Institution: Hokkaido University
• Principal Investigator: KAZUMATA Ken 北海道大学, 大学病院, 講師 (60634144)
• Co-Investigator(Kenkyū-buntansha): 中山 若樹 北海道大学, 医学研究院, 講師 (40421961) ; 鐙谷 武雄 北海道大学, 大学病院, 助教 (80270726) ; 七戸 秀夫 北海道大学, 大学病院, 准教授 (80374479) ; 寳金 清博 北海道大学, 大学病院, 教授 (90229146)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: もやもや病 / iPS細胞 / 血管内皮細胞

Outline of Final Research Achievements

We differentiated vascular endothelial cells from iPS cells derived from healthy persons and moyamoya disease patients. The expression of cell junction related genes were analysed with microarray and the result showed that CLDN1, DST, NEXN and ITGB3 were down-regulated in moyamoya disease. RT-PCR showed that ITGB3 was significantly downregulated in moyamoya disease, however, there were no significat difference in the expression of DST and NEXN. We could not observe the band intensity of CLDN1 in neither RT-PCR and western blotting. In permeability assay, permeability was increased in endothelial cells of moyamoya disease when Y-27632 was added to medium, whereas permeability decreased in normal control.

Free Research Field

脳血管障害