New BNCT against brain tumor stem cell with BSH-peptide

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K10333
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Neurosurgery
• Research Institution: Okayama University
• Principal Investigator: Michiue Hiroyuki 岡山大学, 中性子医療研究センター, 准教授 (20572499)
• Co-Investigator(Renkei-kenkyūsha): KITAMATSU Mizuki 近畿大学, 理工学部, 講師 (60379716)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: ホウ素中性子捕捉療法（BNCT） / ホウ素製剤 / 細胞膜通過ペプチド / 陽電子放射断層撮影（ PET）

Outline of Final Research Achievements

We already succeeded to create new boron compound with cell penetrating peptide (CPP) for boron neutron capture therapy (BNCT). The CPP fused multi-BSH was synthesized in previous project, but it was very difficult to apply to high-volume synthesis for clinical use. In this time, we made up new BSH-CPP with possibility of clinical application and showed new boron drug imaging system for pharmacokinetic evaluation.
A) We made up BSH-3R with fused minimum number of CPP, 3R for clinical use. B) We observed intra-cellular localization of BSH-3R in human glioma cells and finally this boron compound localized at the nucleus of glioma cells. C) We created new positron emission tomography (PET) probe, BSH-3R-DOTA-64Cu. This BSH-3R-DOTA-64Cu was consisted of boron compound part (BSH), CPP part (3R), metal chelate part (DOTA, 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid) and radioactive substance part (64Cu).

Free Research Field

悪性脳腫瘍