2017 Fiscal Year Final Research Report
Establishment of osteosarcoma stem cell model induced by RAGE and elucidation of its molecular mechanism
| Project/Area Number |
15K10435
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Kanazawa University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
山本 靖彦 金沢大学, 医学系, 教授 (20313637)
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| Co-Investigator(Renkei-kenkyūsha) |
MUNESUE Seiichi 金沢大学, 医学系, 助教 (10399040)
HARASHIMA Ai 金沢大学, 医学系, 助教 (50705522)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | 骨肉腫 / がん幹細胞 |
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| Outline of Final Research Achievements |
RAGE (receptor for advanced glycation end-products) overexpressing cells in osteosarcoma (HOS-RAGE)) showed the high cell proliferation and migration ability compared to the control (HOS-mock). HOS-RAGE strongly expressed the stem cell marker (NANOG and SOX2). Microarray analysis of gene expression profiling showed the upregulation of MYC (oncogene). Microarray analysis of miRNA expression profiling showed the downregulation of a certain miRNA. It has been reported that a certain miRNA regulated the expression of pluripotency genes such as SOX2, NANOG and N-MYC in other cancers. Our results suggest the RAGE could suppress the expression of a certain miRNA and may promote the cancer stem-like cell characteristics in osteosarcoma.
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| Free Research Field |
整形外科
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