The role of cathepsin K in the bone and lung metastases

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K10438
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Aichi Cancer Center Research Institute (2016-2017); Nagoya University (2015)
• Principal Investigator: KOZAWA EIJI 愛知県がんセンター(研究所), 分子病態学部, 研究員 (60635572)
• Co-Investigator(Kenkyū-buntansha): 西田 佳弘 名古屋大学, 医学系研究科, 准教授 (50332698) ; 浦川 浩 名古屋大学, 医学部附属病院, 病院講師 (60584753) ; 生田 国大 名古屋大学, 医学部附属病院, 医員 (40732657) ; 濱田 俊介 名古屋大学, 医学部附属病院, 医員 (90747289) ; 筑紫 聡 愛知県がんセンター(研究所), 腫瘍免疫学部, 研究員 (90378109)
• Research Collaborator: YOSHIDA Masahiro ; HAYASHI Takuma
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 腫瘍 / カテプシンK / 乳癌 / 癌骨転移 / 遠隔転移 / 細胞・組織

Outline of Final Research Achievements

CatK synthesized by osteoclasts and/or tumor cells, tumor microenvironmental　macrophage/fibroblasts to be an obvious candidate for a key enzyme involved in the destruction of bone matrix. To establish a murine bone metastasis model, a needle with syringe was used to inject MDA-MB-231 cells and Lewis lung cancer cells into the tibia of mice. Osteolytic lesions progressed markedly at tibia of mice. Scion image analysis revealed that the size of the osteolytic increased from 2 to 4 weeks. The expansion of bone lytic areas of tibia with Lewis lung cancer progressed rapidly for 2 weeks. Histological stain for bone tumor was performed.
The sample included 50 patients with lung tumors and 19 patients with musculoskeletal tumors. The overall 5-year survival rate of the patients was 57.7%. Pre-operative MRI evaluation is helpful to determine whether tumors should be resected with adjacent bone or not.

Free Research Field

整形外科