Acceleration of bone repair using miRNA-induced bone marrow cells for osteonecrosis

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K10476
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Hiroshima University
• Principal Investigator: YAMASAKI Takuma 広島大学, 医歯薬保健学研究科(医), 寄附講座准教授 (50444683)
• Co-Investigator(Kenkyū-buntansha): 味八木 茂 広島大学, 病院(医), 講師 (10392490) ; 庄司 剛士 広島大学, 医歯薬保健学研究科(医), 寄附講座助教 (50736569)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 骨壊死 / 単核球 / miRNA / 細胞移植

Outline of Final Research Achievements

MicroRNA (miRNA) was purified from bone marrow aspirated from the patients of steroid-related osteonecrosis of the femoral head. By global analysis of miRNA, overexpression of miR-31, miR-34a, miR-146, miR-210, miR-218 were detected. In order to evaluate the potential of bone formation, each miRNA was administrated to bone differentiation-inducing medium and bone marrow-derived cells were cultured. These 5 miRNAs induced overexpression of collagen type 1A1, and miR-31, miR-146, miR-210, miR-218 induced overexpression of Runx 2. Moreover, each miRNA was administrated to pseudarthrosis in rat and was proven to accelerate bone formation by micro CT evaluation. Induction of appropriate microRNA may have a potential of acceleration of bone formation with participating autocrine effect of miRNA.

Free Research Field

整形外科学