2017 Fiscal Year Final Research Report
Roles of Atoh8 in differentiation of osteoblasts and adipocytes
Project/Area Number |
15K10486
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Orthopaedic surgery
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Research Institution | Kagoshima University |
Principal Investigator |
MAEDA Shingo 鹿児島大学, 医歯学総合研究科, 特任准教授 (60353463)
|
Co-Investigator(Kenkyū-buntansha) |
河村 一郎 鹿児島大学, 医学部・歯学部附属病院, その他 (90535832)
小宮 節郎 鹿児島大学, 医歯学域医学系, 教授 (30178371)
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Project Period (FY) |
2015-04-01 – 2018-03-31
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Keywords | Atoh8 / 骨芽細胞 |
Outline of Final Research Achievements |
Number and bone-forming activity of osteoblasts in bone marrow decreases in aging bone, which leads to osteoporosis. However, the mechanism is not fully understood. We analyzed function of Atoh8, a novel direct target gene of canonical bone morphogenetic protein (BMP) signaling, in osteoblasts in vivo and in vitro.siRNA-mediated loss of Atoh8 promoted osteoblast differentiation, however, bone formation parameters were not altered in Atoh8 knockout (KO) mice, assessed by micro-CT and bone morphohistometry analysis.Instead, osteoclast number and bone resorption was increased in KO mice bone, and bone volume was significantly reduced.Rankl/Opg expression ratio in KO osteoblast was increased which enhanced differentiation of osteoclasts.
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Free Research Field |
骨代謝学、整形外科学
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