Investigation of neuronal activity in the central nervous system and flight reaction induced by acute or chronic nociceptive stress

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K10500
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: University of Occupational and Environmental Health, Japan
• Principal Investigator: OHNISHI Hideo 産業医科大学, 医学部, 非常勤医師 (20279342)
• Co-Investigator(Kenkyū-buntansha): 上田 陽一 産業医科大学, 医学部, 教授 (10232745)
• Co-Investigator(Renkei-kenkyūsha): SAKAI Akinori 産業医科大学, 医学部, 教授 (90248576) ; MORI Toshiharu 産業医科大学, 医学部, 非常勤医師 (80525444) ; KAWASAKI Makoto 産業医科大学, 医学部, 講師 (40644860)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 視床下部 / 侵害刺激 / 脊髄 / ノックアウトマウス / Fos / FosB / TRPV1 / TRPV4

Outline of Final Research Achievements

We investigated Fos protein or FosB protein expression as markers of neuronal activity in the central nervous system after various nociceptive stimuli in wild type, TRPV1 knockout, TRPV4 knockout, and TRPV1V4 knockout mice. We also evaluated flight reaction against volatilized formalin in these mice. The results suggested that TRPV1 is involved in neuronal activity in the PVN after acute nociceptive stimuli. In the postoperative pain model, it is suggested that TRPV1 and TRPV4 are involved in neuronal activity in laminae III-IV of the dorsal spinal cord. There was no relevance between TRPV1/TRPV4 and FosB protein expression in laminae I-II of the dorsal spinal cord after chronic nociceptive stimuli caused by type II collagen antibody induced arthritis. Moreover, there was no difference among flight reaction of these mice against volatilized formalin.

Free Research Field

整形外科