Novel regulatory mechanism of anesthetic efficay by GABA-A receptor trafficking

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K10503
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Anesthesiology
• Research Institution: Hirosaki University
• Principal Investigator: UENO Shinya 弘前大学, 医学研究科, 教授 (00312158)
• Co-Investigator(Kenkyū-buntansha): 尾崎 拓 弘前大学, 医学研究科, 特任助教 (70621069)
• Research Collaborator: FURUKAWA Tomonori 弘前大学, 大学院医学研究科, 助教 (60402369) ; NIKAIDOU Yoshikazu 弘前大学, 大学院医学研究科, 助教 (50613478) ; SHIMOYAMA Shuji 弘前大学, 大学院医学研究科, 助教 (60736370)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 麻酔作用調節 / GABA-A受容体トラフィッキング / 受容体リン酸化調節

Outline of Final Research Achievements

We showed that efficacy of anesthetics was regulated by GABA-A receptor trafficking via receptor phosphorylation. GABA-A receptor is the major target protein of anesthetics and distributed synaptic and extrasynaptic sites of plasma membrane. Disruption of trafficking of these receptors from synaptic to extrasynaptic sites caused to reduce the efficacy of anesthetics. Interesingly, this reduced effect was rescued in the presence of dephosphorylation inhibitor. These results indicated that the levels of phosphorylation in the receptor determined the efficacy of anesthetics, suggesting that efficacy of anesthetics is controlable even after administration of anesthetics.

Free Research Field

神経科学