Effects of Anesthetics on TRPC channel mediated myocardial protection

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K10534
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Anesthesiology
• Research Institution: Shiga University of Medical Science
• Principal Investigator: Hirotoshi KITAGAWA 滋賀医科大学, 医学部, 教授 (50252391)
• Co-Investigator(Renkei-kenkyūsha): YAMAZAKI TOJI 滋賀医科大学, 医学部, 非常勤講師 (20116122) ; KOJIMA AKIKO 滋賀医科大学, 医学部, 助教 (50447877) ; MATSUURA HIROSHI 滋賀医科大学, 医学部, 教授 (60238962) ; TAKAHASHI KAN 滋賀医科大学, 医学部, 准教授 (80346014)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 吸入麻酔薬 / TRPCチャネル / 心筋保護 / 虚血再灌流傷害 / マイクロダイアリシス

Outline of Final Research Achievements

Using microdialysis technique, we monitored myocardial interstitial myoglobin in the ischemic region of anesthetized rat during ischemia and after reperfusion. In the vehicle, myoglobin levels increased during ischemia and after reperfusion. 2-APB, SKF96365, a TRPC channel blocker, suppressed the increase in dialysate myoglobin levels during ischemia and reperfusion. Sevoflurane also reduced dialysate myoglobin levels during ischemia and reperfusion. In transverse aortic constriction (TAC) model rats with TRPC channel activation, myoglobin levels also increased during ischemia and after reperfusion. These were further suppressed than those in wild type rats with pretreatment of 2-APB, SKF96365 and sevoflurane. These results suggested that TRPC channels plays a significant role in cardiomyocyte injury during ischemia and after reperfusion. Furthermore, sevoflurane might exert an in vivo myocardial protective effect during ischemia and after reperfusion via TRPC channels.

Free Research Field

周術期管理学