Expression profiling of microRNAs in seminal plasma as a new tool for diagnosis of spermatogenic disorder

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K10652
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Urology
• Research Institution: St. Marianna University School of Medicine
• Principal Investigator: Yoshiike Miki 聖マリアンナ医科大学, 医学部, 研究技術員 (60398964)
• Co-Investigator(Kenkyū-buntansha): 岩本 晃明 国際医療福祉大学, 大学病院, 教授 (60046117)
• Research Collaborator: Kosaka Nobuyoshi ; Nozawa Shiari
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 造精機能障害 / マイクロRNA

Outline of Final Research Achievements

This study aimed to assess the utility of microRNAs (miRNAs) as new biomarkers for diagnosis of spermatogenic disorder and clinical or basic research of andrology.
We found the miRNAs showing altered expression specifically in patients with impaired spermatogenesis based on expression profiles of in seminal plasma. Finally, we generated an expression panel with 8 miRNAs related to impaired spermatogenesis through comprehensive microarray analysis of candidate miRNAs.
Determination of miRNA expression levels in seminal plasma with real-time PCR revealed that miR-891a-5p was significantly decreased in the patients with obstructive azoospermia, and miR-1229-3p was significantly increased in the patients with germ cell maturation arrest.

Free Research Field

生殖医学