2017 Fiscal Year Final Research Report
Analyses of morphology and function for tricellular junction in human nasal epithelial cells
| Project/Area Number |
15K10788
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Sapporo Medical University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | human nasal epithelium / epithelial barrier / tricellulin / LSR / Capcisin |
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| Outline of Final Research Achievements |
In the present study, we investigated the roles of epithelial barrier at tricellular contact of human nasal epithelium by analyses of morphology and function for tricellular junctions. In normal human nasal mucosa tissues, tricellulin and LSR regulated the tricellulin and tricellular contact, expressed at the apicalmost of nasal epithelium.In PCR analysis, not only tricellulin and LSR but also ASPP2 family ASPP1 and ASPP2 interacted with LSR, and PAR3 bind ASPP2 were observed in normal human nasal mucosa tissues.Knockdown of tricellulin or LSR by the siRNAs decrease the epithelial barrier function.When the nasal epithelial cells were treated with Capcisin ,Capcisin induced the localization change of LSR from bicellular region to tricellualr region. Taken together, tricellular tight junction molecules tricellulin and LSR formed tricellular junctions may play crucial roles of human nasal epithelial barrier via the interactions with various tight junction-related molecules.
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| Free Research Field |
鼻科学
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