in age related macular degeneration

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K10874
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Ophthalmology
• Research Institution: Nagoya City University
• Principal Investigator: AKI KATO 名古屋市立大学, 大学院医学研究科, 講師 (60405157)
• Co-Investigator(Kenkyū-buntansha): 安川 力 名古屋市立大学, 大学院医学研究科, 准教授 (00324632)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 加齢黄斑変性 / 網膜色素上皮 / ブルッフ膜

Outline of Final Research Achievements

Human retinal pigment epithelium (RPE) cells were cultured without growth factor. For making spheroids, human RPE cells were trypsinized, collected, and resuspended with methylcellulose solution in round-bottom culture plate. Spheroids were collected every day and fixed in in 4% paraformaldehyde, thereafter, the expression of actin was evaluated by immunohistochemistry. Suspended human RPE cells with methylcellulose solution in round-bottom culture plate has been imaged in long-term time-lapse movies over 48 hours. The shape and size of spheroids were analyzed at each time point. The effect of Rho-associated kinase (Rho-kinase) inhibitor for spheroids was evaluated by time-lapse imaging and expression of associated protein.

Free Research Field

網膜硝子体