Clinical application of photodynamic antimicrobial chemotherapy for infectious keratitis

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K10894
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Ophthalmology
• Research Institution: Hiroshima University
• Principal Investigator: Chikama Taiichiro 広島大学, 医歯薬保健学研究科(医), 准教授 (00263765)
• Co-Investigator(Kenkyū-buntansha): 高 知愛 広島大学, 医歯薬保健学研究科(医), 准教授 (70314797)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 光線力学療法 / 角膜感染症 / 抗病原微生物 / 薬剤耐性

Outline of Final Research Achievements

The aim of this study is to establish a new treatment with photodynamic antimicrobial chemotherapy (PACT) for infectious keratitis.
In this period, we evaluated we evaluated the effectiveness of photodynamic antimicrobial chemotherapy (PACT) with TONS504 on filamentous fungi and Acanthamoeba. TONS504-PACT inhibited the growth of Fusarium. solani. However, there was no inhibitory effect for Aspergillus fumigatus by TONS504-PACT. TONS504-PACT suppressed the viability of Acanthamoeba through apoptosis and necrosis in vitro.
We have started to evaluate the in vivo effectiveness of TONS504-PACT. PACT with TONS504 attenuated the development of corneal infection with S. aureus in a rabbit model in vivo, with repeated application of PACT improving its efficacy.

Free Research Field

眼科学