Microbiome analysis of apical periodontitis and cellulitis in oral cavity

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K11023
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Morphological basic dentistry
• Research Institution: Tokyo Dental College
• Principal Investigator: ISHIHARA Kazuyuki 東京歯科大学, 歯学部, 教授 (00212910)
• Co-Investigator(Kenkyū-buntansha): 村松 敬 東京歯科大学, 歯学部, 准教授 (00276982) ; 菊池 有一郎 東京歯科大学, 歯学部, 助教 (30410418) ; 柴山 和子 東京歯科大学, 歯学部, 講師 (60408317) ; 国分 栄仁 東京歯科大学, 歯学部, 講師 (70453785)
• Co-Investigator(Renkei-kenkyūsha): YAMASHITA Yoshihisa 九州大学, 歯学部, 教授 (20192403) ; MURAMATSU Kyotaro 東京歯科大学, 歯学部, 助教 (90507328)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 根尖性歯周炎 / マイクロバイオーム / 16S rRNA pyrosequencing / Prevotella / Porphyromonas / Fusobacterium / Parvimonas / Polymicrobial infection

Outline of Final Research Achievements

In the lesion of apical periodontitis, approximately 55 species of bacteria were detected. Among them, predominant genera were Prevotella, Porphyromonas, Parvimonas, Peptostreptococcus, Fusobacterium. The microbiome was different between the lesion in single space and the lesion with multi space. We also detected the difference in microbiome between the lesion with bleeding and the lesion with discharge of pus. These data suggest the association between the clinical condition and microbiome of the lesion.

Free Research Field

細菌学