2017 Fiscal Year Final Research Report
Hypoxia-responsive MYC promotes the survival and growth of pleomorphic adenoma cells in hypoxic conditions.
| Project/Area Number |
15K11069
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Pathobiological dentistry/Dental radiology
|
|---|
| Research Institution | Niigata University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
阿部 達也 新潟大学, 医歯学総合研究科, 特任助教 (70634856)
山崎 学 新潟大学, 医歯学系, 助教 (10547516)
程 クン 新潟大学, 医歯学系, 准教授 (40207460)
朔 敬 大阪歯科大学, 歯学部, 客員教授 (40145264)
|
|---|
| Project Period (FY) |
2015-04-01 – 2018-03-31
|
| Keywords | 唾液腺多形性腺腫 / 低酸素 / HIF-1α / c-Myc / SM-AP |
|---|
| Outline of Final Research Achievements |
On the basis of hypovasucularity of the salivary pleomorphic adenoma, we had a hypothesis that pleomorphic adenoma cells are able to survive in hypoxic conditions. To understand the hypoxia-dependent manner of energy metabolism in this particular tumor, we analyzed function of MYC, which are the most important factor of metabolic regulation, in cell proliferation and migration in hypoxic-conditioned pleomorphic adenoma cells. In hypoxic condition, SM-AP cells, human pleomorphic adenoma cell systems, showed higher gene expression levels of HIF-1α and MYC in hypoxia. HIF-1α protein was also kept in higher levels and localized more significantly in nuclei. The proliferation and migration of SM-AP cells were reduced under the lack of MYC expression. These results indicated that hypoxic conditions induced pleomorphic adenoma cells to produce MYC, which was maintained by high HIF-1α protein levels.
|
| Free Research Field |
医歯薬学
|
