Establishment of dental pulp tissue engineering by using autologous dental pulp stem cells in rat molars

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K11110
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Conservative dentistry
• Research Institution: Tokyo Medical and Dental University (2016-2017); Niigata University (2015)
• Principal Investigator: KANEKO Tomoatsu 東京医科歯科大学, 大学院医歯学総合研究科, 助教 (70345297)
• Research Collaborator: Nör Jacques E. 米国ミシガン大学, 歯学部う蝕学・歯内治療学, 教授 ; Jin Qiming 米国ミシガン大学, 歯学部歯周病学, 講師
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 歯髄再生

Outline of Final Research Achievements

In present analysis was performed by using in vivo protocol for pulp tissue engineering in pulpotomized rat teeth using implantation of stem cells. This model showed complete regeneration/healing with dentin bridge like formation within 2 weeks. First, dental pulp stem cells and endothelial cells from rat molars or incisors were corrected and cultured. Second, in vitro co-culture was performed and we found VEGF is one of key factors of cell growth of stem cell and/or endothelial cells. Finally, the implantation of stem cells and endothelial cells treated with VEGF into rat molars was performed. This model showed dental pulp tissue regeneration/healing within 1 week.

Free Research Field

歯内療法学