Regeneration of dental pulp/dentine composite by oral mucosal cells in novel hybrid scaffold

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K11140
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Conservative dentistry
• Research Institution: Osaka Dental University
• Principal Investigator: KAKIGI Hideyuki 大阪歯科大学, 歯学部, 講師(非常勤) (40642830)
• Co-Investigator(Kenkyū-buntansha): 前田 博史 大阪歯科大学, 歯学部, 教授 (00274001) ; 好川 正孝 大阪歯科大学, 歯学部, 客員准教授 (70148451)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 口腔粘膜細胞 / Dexamethasone / β-Glycerophosphate / 二相性担体 / In vivo / In vitro

Outline of Final Research Achievements

Addition of dexamethasone in culture medium of rat oral mucosal cells obtained induced small amount of calcified nodule deposition. The quantity was too small in comparison with bone marrow cells. This result suggested the possibility that oral mucosal cells might dedifferentiate to hard tissue forming cells. The factor which induced the proliferation of oral mucosa cells and those re-differentiation to hard tissue forming cells was examined. And cyanocobalamin revealed an effect.
Oral mucosa cells with seeding of a sponge made from alginic acid inserted in the cylindrical porous hydroxyapatite scaffold with a hollow center. In the subcutaneously implanted biphasic scaffold, a small amount of osteocalcin was detected. Submucosal connective tissue cells might differentiate to osteogenic cells.

Free Research Field

歯内治療