Bone regeneration using a hybrid-type artificial bone consisted of iPS cells and low crystalline carbonate apatite

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K11315
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Tohoku University (2016-2017); The University of Tokushima (2015)
• Principal Investigator: Nagai Hirokazu 東北大学, 歯学研究科, 准教授 (50282190)
• Co-Investigator(Kenkyū-buntansha): 宮本 洋二 徳島大学, 大学院医歯薬学研究部(歯学系), 教授 (20200214) ; 玉谷 哲也 徳島大学, 病院, 講師 (30274236) ; 藤澤 健司 徳島大学, 病院, 講師 (40228979) ; 大江 剛 徳島大学, 病院, 助教 (60432762)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 骨再生医療 / 炭酸アパタイト / iPS細胞

Outline of Final Research Achievements

In this study, we try to develop the bone regenerative therapy using a hybrid-type artificial bone consisted of human induced pluripotent stem (iPS) cells and low crystalline carbonate apatite (CAp). We used iPS cells for cell source of bone regenerative therapy, and used low crystalline CAp and collagen complex for scaffold of bone regenerative therapy. As a result, it was revealed that 1) low crystalline CAp and collagen complex implanted into the cranial bone defect of rabbits induced new bone tissues, 2) the differentiation of human iPS cells to mesenchymal stem cell-like cells was induced, 3) mesenchymal stem cells cultured on carbonate apatite disk differentiated into osteoblastic cells.

Free Research Field

医歯薬学