CO2 laser therapy accelerates the healing of ulcers in the oral mucosa

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K11378
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthodontics/Pediatric dentistry
• Research Institution: Fukuoka Dental College
• Principal Investigator: OZAKI MASAO 福岡歯科大学, 口腔歯学部, 教授 (10152472)
• Co-Investigator(Kenkyū-buntansha): 岡 暁子 福岡歯科大学, 口腔歯学部, 准教授 (60452778)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: CO2 laser / Immunohistochemistry / Epithelial tissue / CO2 laser / wound healing / tenascin C

Outline of Final Research Achievements

The present study used a mouse model of ulceration to investigate the molecular mechanisms by which CO2 laser therapy accelerated the wound healing process.Immunohistochemistry experiments showed that heat shock protein-70 (HSP70) was expressed mainly in the epithelium of normal palatal tissue, whereas there was little tenascin C (TnC) expression in the epithelium and mesenchyme under normal conditionsLaser irradiation induced HSP70 mRNA and protein expression in the lamina propria as well as TnC expression in the mesenchyme underlying the renewing epithelium.Culture of fibroblasts in heated medium increased the expressions of both TnC and TGF-β1, whereas laser irradiation induced only TnC expression.The present study indicates that CO2 laser irradiation exerts a photobiogenic effect to upregulate TnC expression without inducing TGF-β1 expression.We suggest that CO2 laser therapy has an advantage over thermal stimulation.

Free Research Field

小児歯科学