Physiological role of mechanical stress in alveolar bone resorption induced by the oral malodorous compounds.

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K11432
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Social dentistry
• Research Institution: The Nippon Dental University
• Principal Investigator: IMAI TOSHIO 日本歯科大学, 生命歯学部, 教授 (90120617)
• Co-Investigator(Kenkyū-buntansha): 那須 優則 日本歯科大学, 生命歯学部, 教授 (50130688)
• Co-Investigator(Renkei-kenkyūsha): YAEGAKI KEN 日本歯科大学, 生命歯学部, 教授 (40166468) ; II HISATAKA 日本歯科大学, 生命歯学部, 講師 (00746604)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 口臭物質 / 歯槽骨吸収 / メカニカルストレス / 破骨細胞 / 骨芽細胞 / 細胞内シグナル伝達

Outline of Final Research Achievements

It was aimed to elucidate the effect of mechanical stress on alveolar bone resorption by the oral malodorous compounds (hydrogen sulfide). Alveolar bone is routinely exposed to mechanical stress. A stretchable silicone vessel (SW) was used for the stretch stress. After coating SW with Poly-L-Lysine, Fibronectin, Collagen I, osteoclastic cells RAW 264 was seeded and induced the differentiation by RANKL.TRAP positive cells was observed only a little compared to the plastic dish.Cell proliferation and differentiation of osteoblastic cells MC3T3-E1 was observed in SW corted with Matrigel. It was suggested that ERK 1/2 plays an important role in its mechanism when hydrogen sulfide treated MC 3 T 3 - E 1 is on the stretch stress.

Free Research Field

生理学