Construction of screening method of epi-mutagens based on control of intracellular DNase I

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K12214
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Risk sciences of radiation and chemicals
• Research Institution: University of Shizuoka
• Principal Investigator: Ibuki Yuko 静岡県立大学, 食品栄養科学部, 教授 (30236781)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: エピジェネティクス / DNase I / 界面活性剤 / ヒストン / クロマチン構造 / 熱 / γ-H2AX

Outline of Final Research Achievements

We found that some kinds of surfactants like nonylphenol polyethoxylates and linear alkylbenzene sulfonates induced phosphorylation of histone H2AX (γ-H2AX ) via release of free deoxyribonuclease I (DNase I) with filamentous actin disruption. DNase I was translocated from the cytosol to the nucleus of cells after treatment with surfactants, and generated DNA strand breaks. The surfactant-induced generation of γ-H2AX increased relative to the acetylation caused by the treatment with histone deacetylase inhibitor, suggesting that the change of chromatin structure induced by environmental chemicals might be detectable by surfactant-induced γ-H2AX. On the other hand, we also found that heat stress induced similar release of DNase I and generation of γ-H2AX. This might be useful to establish the evaluation method for chromatin structure.

Free Research Field

毒性学