Transformation and recognition of shape of lipid membrane by amphiphilic DNA nanostructures

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K12746
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biomolecular chemistry
• Research Institution: Osaka University
• Principal Investigator: Dohno Chikara 大阪大学, 産業科学研究所, 准教授 (60420395)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: DNA / DNAナノ構造 / 両親媒性DNA / 膜変形 / 脂質二重膜 / リポソーム

Outline of Final Research Achievements

Lipid membranes play an important role in biological system. The lipid membranes define the boundaries of cell and various organelles, and their shape and physical properties are dynamically controlled in the living cells. We here propose a methodology to control the shape and mechanical properties of the lipid membranes by using nanoscale DNA objects made of membrane binding DNAs. We created a series of DNA nanostructures with various 2- and 3-dimensional shapes that efficiently bind to lipid membrane by introducing amphiphilic DNA strands containing hydrophobic functional groups. We demonstrated the binding of the DNA nanostructures could change a shape of liposome and mechanical properties of the lipid membranes.

Free Research Field

生物有機化学