2016 Fiscal Year Final Research Report
Development of DEP device for analysis of mechanobiology that affects cell function and differentiation
| Project/Area Number |
15K13721
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Analytical chemistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Keywords | 細胞分析 / 誘電泳動 / 幹細胞 / メカノバイオロジー |
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| Outline of Final Research Achievements |
Although mechanical stimulation to the cells is recognized as an important factor to control cellular functions, conventional methods for cell stimulation are difficult to control the intensity and timing of mechanical stimulation with simple procedures and equipment.
In this study, we investigated the effect of positive dielectrophoresis (DEP) on gene expression in mesenchymal stem cells. When applying the alternating current voltage, human bone marrow derived mesenchymal stem cells (UE7T-13) exhibited positive DEP and were compressed onto the electrode surface. Constructed device can easily control the DEP force to the cells by changing frequency. Interestingly, gene expressions of cell differentiation marker in UE7T-13 cells and mechanical stimulation-susceptible one were changed by applying positive DEP. These results suggested that gene expression in mesenchymal stem cells can be regulated by applying mechanical stimulation derived from DEP.
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| Free Research Field |
バイオ分析
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